Protein Concentration Estimation
gends at leeds.ac.uk
Mon Jul 18 08:37:00 EST 1994
I need an accurate method of measuring protein concentration. My sample is an
RNA binding protein, and for various reasons very difficult to get rids of the
nucleotide without also getting rid of your sample. The prescence of the
nucleotide is not inhibitory on the studies I'm carrying out, but it is
providing just a few problems in estimating how much sample is there.
Absorbance provides anomalous answers, and Biorad (Bradford) assays are out as
the answer needs to be accurate to within 5%. Amino Acid analysis tells us
accurately how much protein is there, but I'd love a way of doing this in less
than two days!
So is there any ideas out there? Has anyone come across similar problems,
pointers in the right direction and information on dead ends not to pursue
would be very much appreciated!
Cheers in advance,
Dept of Genetics.
University of Leeds.
Email: gends%south-01.novell.leeds.ac.uk at gps.leeds.ac.uk
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