Phosphorylation of Proteins - Detection?

riadl3261 at cobra.uni.edu riadl3261 at cobra.uni.edu
Mon Jul 18 12:55:06 EST 1994


>>In article <2vitui$q7q at dingo.cc.uq.oz.au>, forrest at biosci.uq.oz.au
> (acer) wrote:
>>> G'day all,
>>>         Does anyone know of a NON-radioactive procedure for testing
> whether a 
>>> protein is phosphorylated or not? 
> 
And...
>>
>       Western blot after immunoprecipitation (or not) would be the
> non-radioactive method of choice if you have little material to start
> with. Unfortunately, commercial anti-phosphotyrosine antibodies often
> display quite different specificity. And antiphosphoserine and -thr are
> sold by Sigma, but I have not seen lately any publication mentionning
> their use ??
>      > 	Strasbourg	France
> 	hubert at cigale.u-strasbg.fr

WE have used a monoclonal antiphosphoserine made by Sigma and it has worked
very well on western blots.  We used the antibody to detect phosphorylated
connexin proteins and the results were good.

LMR



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