Protein Concentration Estimation
Mon Jul 18 13:19:52 EST 1994
In article <gends.6.000D6D00 at leeds.ac.uk>, gends at leeds.ac.uk (SCOTT D.)
> I'm trying to estimate the concentration of a protein sample that is
> contaminated with nucleotide (it binds RNA, and the nucleic acid is near
> impossible to get rid of without getting rid of your sample).
> Any ideas out there??? Tried Biorad, but I need accuracy better than 5%, and
> obviously Absorbance is a bit tricky with nucleic acid present.
> Help much appreciated!
> Dave Scott.
> Dept Of Genetics,
> University of Leeds.
> Email: gends%south-01.novell.leeds.ac.uk at gps.leeds.ac.uk
Hope this will help:
Stevens, L. (1992) Buffers and the determination of proetin concentrations.
from "Enzyme Assays", R. Eisenthal and M. J. Danson, eds. IRL Press,
Oxford University Press.
It contains quite a few methods in it. A nice review.
ingnang at sbbiovm.sunysb.edu
Dept. Ecology and Evolution
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