Best way to adjust pH of a protein?
jcburd at aol.com
Sat Jul 23 23:02:07 EST 1994
In article <1994Jul18.184220.22973 at alw.nih.gov>, Jim Owens
<jow at helix.nih.gov> writes:
Use a 0.1M concentrate of a weak acid of base (i.e. tris or acetate). pH
Adjust the concentrate to avoid extremes of pH. I like to use acetate at
pH 4. The higher salt concentation can be lowered by using a Ion retarding
column (available frm Bio rad or Sterogene). Or if volume is not a problem
use water or lower concentrated buffer.
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