Fluorescent protein labeling

Steven Striepeke sstriepk at crl.com
Tue Jul 26 10:57:59 EST 1994


Has anyone tried using o-pthalaldehyde to fluorescently label proteins 
and then run them on a gel?

I've heard that the fluorescent conjugate is short lived (approx 2 hr.s) 
but I haven't gotten a good explanation about what the nature of the 
instability is. Is it photo-bleaching or some other sort of breakdown.


Steve Striepeke
sstriepk at crl.com



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