Fluorescent protein labeling

Steven Striepeke sstriepk at crl.com
Wed Jul 27 17:47:32 EST 1994


The labeling reaction involves the formation of a fluorescent structure 
by conjugating the o-pthalaldehye with a primary amine on a protein and 
the thiol of mercaptoethanol in the presence of sodium thiocyanate, 
ethyl acetate, methanol, and Brij-35 in water.

Pierce sells it as Fluoraldehyde Protein\Peptide assay reagent

Steve
sstriepk at crl.com


Steve Rogers 
(steve_rogers at qms1.life.uiuc.edu) wrote:
: In article <313bq7$m79 at crl3.crl.com>, sstriepk at crl.com (Steven Striepeke)
: wrote:

: > Has anyone tried using o-pthalaldehyde to fluorescently label proteins 
: > and then run them on a gel?
: > 
: > I've heard that the fluorescent conjugate is short lived (approx 2 hr.s) 
: > but I haven't gotten a good explanation about what the nature of the 
: > instability is. Is it photo-bleaching or some other sort of breakdown.
: > 
: > 
: > Steve Striepeke
: > sstriepk at crl.com


: Could someone post a reference describing this conjugation?  Sounds
: interesting, but I'm too lazy to do a search.  Thanks.

: -- 
: "A well-done experiment gives the same sense of satisfaction that a
: composer feels after composing a sonata."  --Hilary Koprowsky

: Steve Rogers
: Dept. of Cell & Structural Biology
: Univ. of Illinois @ C-U
: steve_rogers at qms1.life.uiuc.edu



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