Fluorescent protein labeling

[Steve Rogers]

In article <313bq7$m79 at crl3.crl.com>, sstriepk at crl.com (Steven Striepeke)
wrote:

> Has anyone tried using o-pthalaldehyde to fluorescently label proteins 
> and then run them on a gel?
> 
> I've heard that the fluorescent conjugate is short lived (approx 2 hr.s) 
> but I haven't gotten a good explanation about what the nature of the 
> instability is. Is it photo-bleaching or some other sort of breakdown.
> 
> 
> Steve Striepeke
> sstriepk at crl.com


Could someone post a reference describing this conjugation?  Sounds
interesting, but I'm too lazy to do a search.  Thanks.

-- 
"A well-done experiment gives the same sense of satisfaction that a
composer feels after composing a sonata."  --Hilary Koprowsky

Steve Rogers
Dept. of Cell & Structural Biology
Univ. of Illinois @ C-U
steve_rogers at qms1.life.uiuc.edu