Good MW Markers for Silver Staining

Alan Kaiser alkaiser at eden.rutgers.edu
Thu Jul 28 16:07:19 EST 1994


In <edbeaty-240794192301 at koniskyj3.life.uiuc.edu> edbeaty at uxa.cso.uiuc.edu (ed beaty) writes:

>Hi,

>Our lab is purifying a number of proteins for N-terminal sequencing and
>other applications.  We usually silver-stain our gels to make sure there
>are no contaminating proteins which could interfere with our sequencing. 
>The problem is that our MW standards (from Sigma) are designed for
>Coomassie Blue staining, and give many strong "mystery bands" when we
>silver stain them.  I assume this is because the proteins used to make the
>standards aren't too pure to begin with.  We've tried diluting the
>standards more to make the mystery bands fainter, but some of these bands
>are nearly as strong as the expected bands.  


>Does anyone know of a cleaner set of MW standards that are commercially
>available and give (reasonably) clean bands on a silver stained gel (i.e.
>good enough to send to a reviewer without having to say, "okay, ignore
>these twelve bands; that's BSA, that's lysozyme...or is it THAT band...")

>Thanks fer yer help.

>Ed Beaty
>edbeaty at uxa.cso.uiuc.edu

Try the silver stain standards in the Sigma catalog (p. 1751, 1994
catalog). I have not personally used these standards but I know that
you can not use normal standards with silver stain and expect nice
results. 

If anyone knows of other sources for silver stain markers please let
me know. I am in search of markers suitable for silver stain in a low
molecular weight range (2500 - 10000). Post to this group or send me
e-mail.

Alan Kaiser
alkaiser at eden.rutgers.edu



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