Good MW Markers for Silver Staining
Ekramoddoullah, Abul Kalam M.
aekramoddoul at PFC.Forestry.CA
Fri Jul 29 17:52:48 EST 1994
In article <edbeaty-240794192301 at koniskyj3.life.uiuc.edu>, edbeaty at uxa.cso.uiuc.edu (ed beaty) writes:
>Our lab is purifying a number of proteins for N-terminal sequencing and
>other applications. We usually silver-stain our gels to make sure there
>are no contaminating proteins which could interfere with our sequencing.
>The problem is that our MW standards (from Sigma) are designed for
>Coomassie Blue staining, and give many strong "mystery bands" when we
>silver stain them. I assume this is because the proteins used to make the
>standards aren't too pure to begin with. We've tried diluting the
>standards more to make the mystery bands fainter, but some of these bands
>are nearly as strong as the expected bands.
>Does anyone know of a cleaner set of MW standards that are commercially
>available and give (reasonably) clean bands on a silver stained gel (i.e.
>good enough to send to a reviewer without having to say, "okay, ignore
>these twelve bands; that's BSA, that's lysozyme...or is it THAT band...")
>Thanks fer yer help.
Try BioRad's low mol standard at 1:100 dilution and use fresh sample buffer. I
hope that helps.
>edbeaty at uxa.cso.uiuc.edu
EKRAMODDOULLAH, ABUL KALAM M. Title: Research Scientist
Forestry Canada Phone: (604) 363-0600
Victoria, B.C. Internet: AEKRAMODDOUL at A1.PFC.Forestry.CA
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