Chromatofocusing

Joe Mack mack at ncifcrf.gov
Sun Jul 31 13:01:15 EST 1994


In article <199407271956.MAA17986 at net.bio.net> ERNESTO at VM.CC.PURDUE.EDU (Martin Tiznado) writes:
>Hi everyone!!!!!
>Does anyone know if it is possible to perfom a chromatofocusing from low pH to
>high pH. My protein has a really high pI, so I think that I need to go from low
> to high, but I am not sure since all the techiques that I had reviewed until n
>ow are dealing with pH gradients starting from high pH's. Thanks for your help.
>
>                                                Martin.

I don't know what high is in your books, but ampholites start to
hydrolyse above pH9 making it impossible to IEF etc at those pH's
(the pI of the ampholytes drops).

	Joe Mack
	mack at ncifcrf.gov




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