experiences with pET vectors from novagen

Bipin K Dalmia bipin at iastate.edu
Mon Mar 7 08:49:09 EST 1994


i'm looking for +ve and -ve experiences that you have had with the pET
expression vectors from Novagen. more specifically with the N-terminal
his.tag systems. i have to overexpress a vareity of proteins and i have
chosen this system because it offers the advantage of purification in
the presence of denaturants. most proteins overexpressed do form
inclusion bodies don't they!

also, anyone yet tried the new s.tag system from novagen?

bip


-- 
bipin k. dalmia               the other night i was lying on my bed, looking
bipin at iastate.edu             up at the beautiful stars, and i said to myself, 
n2.bkd at isumvs.iastate.edu     'where the F*CK is my ROOF !!'
--



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