Elution of High Affinity PcAb

matiasm at rand matiasm at rand
Mon Mar 7 19:27:34 EST 1994


Hello Netters,
 
I'm a first time user of this medium.  I am having a problem eluting off
PcAb to p53 (Science Mag's "Molecule of the Year") from an antigen affinity
column.  I've tried low pH (2.6) glycine, 3 Molar MgCl2, and combinations
thereof.  I've also tried an electroelution method with no apparent luck.
Any suggestions would be welcome.  This 'b*#ch' of a protein seems to have
a pretty high affinity to our PcAb.  Thanks in advance!

Matt "AbboGrunt"




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