Apparent MW of protein on-PAGE gel > real MW?

ZHAOMIN 'THE VICTIM OF SCIENCE' ez007215 at ALCOR.UCDAVIS.EDU
Tue Mar 8 23:16:17 EST 1994


In article <2lg6vo$9s7 at charm.magnus.acs.ohio-state.edu>, bhuang at magnus.acs.ohio-state.edu (Baohua Huang) writes:
>Hi, everyone.
>
>A fusion protein I expressed in E. coli has a apparent MW on SDS-PAGE gel ~5 kd
>lager than it's calculated MW. This protein is 303 aa residues with a 
>calculated MW of 35 kd but it runs at 40 kd position on SDS-PAGE. The protein 
>has very high content of basic amino acids (32 lysines,  20 arginine and 16 
>histidines). My question is : does high content of basic residues make a 
>protein run slower?
>I would apreciate very much any hints to my question.
>
>Baohua Huang
>Ohio state Univ.

Is it possible that the protein has been modified posttranslationally, such as 
phosphorylation etc.

Zhaomin
UCDavis



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