antibody production in 8M urea

Hanson Lab lab_mac_hanson at
Wed Mar 9 09:58:39 EST 1994

Has anyone ever tried making an antibody with a protein still in an 8M Urea
solution? This protein is really insoluble and wound up in the above, and I

hesitate to run it over a column or any such procedure for fear of losing
entirely.  I would just like to inject as is, in an estimated solution of
0.5 mg/ml. 

would appreciate knowing your outcome.  

Please reply to 
David Ruth		
DCR7 at

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