antibody production in 8M urea

[Hanson Lab]

Has anyone ever tried making an antibody with a protein still in an 8M Urea
solution? This protein is really insoluble and wound up in the above, and I

hesitate to run it over a column or any such procedure for fear of losing
it
entirely.  I would just like to inject as is, in an estimated solution of
about
0.5 mg/ml. 

would appreciate knowing your outcome.  

thanks.
Please reply to 
David Ruth		
DCR7 at crux2.cit.cornell.edu