Prestaining Proteins

Ding Ming ming at ahabs.wisc.edu
Fri Mar 11 23:33:36 EST 1994


In article <2lqs2r$hdl at news.bu.edu>, rocket1 at bu.edu (Richard Near) wrote:

> I am interested in prestaining some proteins to
> serve as markers in sds gels and/or chromatography.
> I realize that this will affect the apparent Mwt, bu
> t, for my crude applications (at least for now), it will
> serve it's purpose.  MY Question: does anyone have a 
> simple protocol for prelableling proteins that should
> be stable in sds and/or urea.
> 


Sigma, Bio-Rad, and quite a few biochemical vendors sell Prestained SDS-MW
Markers. They are not terribly expensive and pretty neat in Western. Why do
you bother to make them by yourself?

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Ding Ming
University of Wisconsin-Madison
Telephone: (608)265-3544     Fax: (608)262-7420
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