Prestaining Proteins

[Pete Dunten]

Protocol for labeling standards


Dissolve stds in 50 mM phosphate buffer, pH 7.5, 4% SDS.

Add excess dansyl chloride (soluble in acetone).

Heat, 5 min at 100 deg.

Add excess bme.

Heat, 5 min at 100 deg.

Load on Pasteur pipette mini-column containing G10 in
  2% SDS and your gel stacking buffer.

Wash off the column in the same buffer.

First off are the dansylated stds, then dansyl-OH.

Viola, labeled stds (yellow under UV).