disrupting e. coli cells

Pete Dunten dunten at sol.bmc.uu.se
Sat Mar 12 12:07:27 EST 1994

3 volumes buffer to 1 gram (wet) cells works pretty well.  
You can see the cell suspension change color when the cells
break.  Or you can put a few microliters on a slide, cover
with a coverslip, and take a look under a microscope.  Intact
cells and broken cell envelopes can be distinguished, so you
can decide when to stop sonicating.  

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