I can't concentrate!

Brian Ellis - lab mckegney at unixg.ubc.ca
Thu Mar 24 13:45:32 EST 1994


Here is problem that i haven't encountered before.  After FPLC i attempt to 
concentrate the fractions in centricon/centriprep tube (MW cutoff 10K), it 
appears that my protein precipitates out of solution.  Has anyone else 
encountered this problem.  The protein is 78 kD in size and is found in native 
state as a tetramer.  




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