I can't concentrate!

[Joe Mack]

In article <Pine.3.89.9403251526.A22537-0100000 at fox.cce.usp.br> szeinfel at FOX.CCE.USP.BR (Rafael N Szeinfeld) writes:
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>	I'm using centricon to concentrate a trypsin derivated from it 
>reaction with a free radical and after concentration we can't find our 
>protein. At first we thought that the membrane was bad but now we think 
>that our protein in suffering autolysis, could it be your case ?
>	
>	Rafael najmanovich

Do you have EDTA? If not heavy metals will chew up your protein.
	Joe Mack
	mack at ncifcrf.gov