Resolving LMW proteins

Ben Davis bjd12 at
Tue Mar 29 09:36:33 EST 1994

ajayaram at wrote:

: Hi there,

: I'm trying to run a 8 kd protein on a SDS get but I'm having some problems
: getting it to show up due to its small size.  I've tried 10, 12 and 15
: percent gels but the but the protein doesn't seem to be there ( the problem is
	(stuff deleted)

I work on a similar sized protein (7.3kD) - try using a 16% gel with glcerol
(I think we use 1-2mL glycerol per gel). I haven't got the recipe to hand,
but this should work.



Ben Davis,
MRC Protein Function and Design,
Cambridge, UK

"They can make me do it, but they can't make me do it with dignity."

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