Resolving LMW proteins

Ben Davis bjd12 at cus.cam.ac.uk
Tue Mar 29 09:36:33 EST 1994


ajayaram at pearl.tufts.edu wrote:

: Hi there,

: I'm trying to run a 8 kd protein on a SDS get but I'm having some problems
: getting it to show up due to its small size.  I've tried 10, 12 and 15
: percent gels but the but the protein doesn't seem to be there ( the problem is
	(stuff deleted)

I work on a similar sized protein (7.3kD) - try using a 16% gel with glcerol
(I think we use 1-2mL glycerol per gel). I haven't got the recipe to hand,
but this should work.

Ben

--
______________________________________________________________________________

Ben Davis,
MRC Protein Function and Design,
Cambridge, UK
______________________________________________________________________________

"They can make me do it, but they can't make me do it with dignity."



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