protein concentration

Suzy Schneider suzy at welchlink.welch.jhu.edu
Thu Mar 31 14:57:04 EST 1994


As per Eric Herbert's request:

I usually begin with approx. 15ml of protein solution in low salt buffer
(50 mM Tris) containing 5% glycerol.  My main goal is to concentrate to 5
mg/ml or more; sometimes I need to change the buffer and/or pH in order to
proceed to the next purification step.  So I would like to keep losses to a
minimum.

blesley at welchlink.welch.jhu.edu



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