alkaline phosphatase, pH?

pesusi at pesusi at
Thu Mar 31 07:58:57 EST 1994

Hi everybody

I have a simple question. Does any of you know the pH optimum for alkaline
phosphatase? Actually I want to know in vitro conditions. Does the protein work
better in diethanolamine than in Tris based buffers? My purpose is to find
suitable conditions for colourdevelopment in immuno-blots.

P.S I would appreciate answers directly to me.


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