alkaline phosphatase, pH?

[pesusi at sara.cc.utu.fi]

Hi everybody

I have a simple question. Does any of you know the pH optimum for alkaline
phosphatase? Actually I want to know in vitro conditions. Does the protein work
better in diethanolamine than in Tris based buffers? My purpose is to find
suitable conditions for colourdevelopment in immuno-blots.
Thanks.

P.S I would appreciate answers directly to me.

Peter
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