preconcentration of protein prior to SDS PAGE

MR CW HO [GEN] mhocw at ccs1.cc.monash.edu.au
Tue May 3 01:27:50 EST 1994


Hello... i am working on plant proteins at the moment. I have trouble trying 
to extract the protein in appropriate amounts to view them in SDS PAGE. I am 
using 0.07% Mercaptoethanol in 10% TCA in acetone. THis should work 
appropriately. After running on multilane gels I would proceed to put them 
through a column of Mono S resins. I need suggestions of any kind.

thank you

Add.: Mhocw at ccs1.cc.monash.edu.au



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