sds page bands; reply

RAMPITSCH at BCRSSU.AGR.CA RAMPITSCH at BCRSSU.AGR.CA
Tue May 3 13:03:55 EST 1994


In reply to:

>I'm afraid I'm not much of a protein person but even I have to run the
>occasional SDS-PAGE gel.  Most of the time this gives no problem but just
>lately the lower molecular weight bands are very fuzzy.  I'm running 10 to
>20% gels PAGE gels.  My hunch is this is a salt problem but why is it only
>appearing now?  I'm using Sigma Trizma base,  is this O.K.?  Speaking of which
>is there any difference between Trizma base and Tris?

>Bill Greenhalf
>Biotechnology Group,

>E-mail Greenhalfw at Newi.ac.uk

Hi Bill: 
	Yes, Trizma is the same as tris, as far as I know. Your problem
of recent fuzzy bands may be caused by your acrylamide stock going off
as it does over time. Is it polymerizing as rapidly as it used to? or
does it take forever? Try a fresh stock and add anion exchange beads to
the stock (they absorb acrylic acid formed over time). Also, are your
buffer stocks still OK? Are you using a Laemmli system? You've probably
thought of most of this, but it's a start.
	cheers, chris.

(PS, BTW you *are* a protein person :-) 

------------------------------------------------------------------------------
 Chris Rampitsch                       |       RAMPITSCH at BCRSSU.AGR.CA
 Dept. Plant Science, Univ. of BC      |       Phone (604) 494-7711
 Vancouver, British Columbia           |       Fax   (604) 494-0755
 Canada V6T 2A2                      _/\_
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