preferred codon usage in E. coli

miller at LSM.NRL.NAVY.MIL miller at LSM.NRL.NAVY.MIL
Tue May 3 16:19:37 EST 1994

Hello everyone,

I am currently trying to overexpress a eukaryotic protein using a T7 RNA 
polymerase promotor system, but am only getting about 1 mg/liter culture of 
expressed protein.  I am looking for ways to optimize this expresAny  
can determine which codons are most frequently used in E. coli.  Any other 
suggestions would be greatly appreciated.

Charles Miller, PhD

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