preferred codon usage in E. coli

[miller at LSM.NRL.NAVY.MIL]

Hello everyone,

I am currently trying to overexpress a eukaryotic protein using a T7 RNA 
polymerase promotor system, but am only getting about 1 mg/liter culture of 
expressed protein.  I am looking for ways to optimize this expresAny  
 
can determine which codons are most frequently used in E. coli.  Any other 
suggestions would be greatly appreciated.

Charles Miller, PhD