preferred codon usage in E. coli

Joe Mack mack at ncifcrf.gov
Wed May 4 08:16:21 EST 1994


In article <0097DE31.958FC4A0.28681 at lsm.nrl.navy.mil> miller at LSM.NRL.NAVY.MIL writes:
>Hello everyone,
>
>I am currently trying to overexpress a eukaryotic protein using a T7 RNA 
>polymerase promotor system, but am only getting about 1 mg/liter culture of 
>expressed protein.  I am looking for ways to optimize this expresAny  
> 
>can determine which codons are most frequently used in E. coli.  Any other 
>suggestions would be greatly appreciated.

For a table of codons look in the Novagen Catalogue, 1994, p68 (Ref Gribskov
et al NAR 1984, 12, 539-49). For an example of increasing expression of a
gene see Holler et al, Gene 1993, 136, 323-8.

Joe Mack, BSc 
mack at ncifcrf.gov

>
>Charles Miller, PhD
>





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