preferred codon usage in E. coli

Eric J Hebert erich at
Wed May 4 16:14:32 EST 1994

In article <CpA4vA.BCA at> mack at (Joe Mack) writes:

>For a table of codons look in the Novagen Catalogue, 1994, p68 (Ref Gribskov
>et al NAR 1984, 12, 539-49). For an example of increasing expression of a
>gene see Holler et al, Gene 1993, 136, 323-8.

>Joe Mack, BSc 
>mack at

>>Charles Miller, PhD

In addition to Joe's comments, I believe 

deBoer, H.A. & Kastelein, R.A. (1986) in Maximizing Gene Expression, eds. 
Rezinkoff, W & Gold, L. (BUtterworth, Stoneham, MA). pp. 225-285.

is an excellent reference.

Also Springer and Sligar have used this information in the "High-Level 
expression of Sperm Whale Myoglobin in E. coli"  in PNAS 84:8961-8965 (1987) 
for an example of its use.

Good Luck


Erich at

More information about the Proteins mailing list