Agarose gels for protein purification?

Holger Berk berk at uxa.cso.uiuc.edu
Thu May 5 16:49:34 EST 1994


Hello!

My question THIS time is about using agarose gel electrophoresis for the
purification of proteins.  Has anyone out there had any experience with
this procedure?  Is it possible to detect the proteins in the agarose gel
by coomassie blue staining, or do you have to transfer the protiens to a
membrane first?  Also, is contamination with proteins present in the
agarose itself a problem?  Any references anyone could send me about this
procedure would be very greatly appreciated!

Thank you,
Ed Beaty

Ed_Beaty at qms1.life.uiuc.edu



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