Immunoaffinity Purification Using An IgM
udbl119 at bay.cc.kcl.ac.uk
Mon May 9 14:10:01 EST 1994
I have a monoclonal antibody which is an IgM and I would like to use it to
purify the protein that it recognises. I think the best way to do
this is by doing an immunoaffinity isolation, linking my antibody to a solid
support column but I do not know how effective it will be with an IgM antibody
as apposed to an IgG. I was wondering if anyone out there has had any
experience linking IgM abs to a column and the best way to go about this.
Also is there a commercially available kit that I can buy that you would
recommend. I have an abundant supply of the monoclonal. Many thanks in
(M.Johnstone at bay.cc.kcl.ac.uk)
King's College, London.
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