Peptide Synthesis

John E. Fox J.E.Fox at bham.ac.uk
Fri May 13 11:51:58 EST 1994


In article <2qtk1j$nnn at usenet.INS.CWRU.Edu>, evw2 at po.CWRU.Edu (Eric V. Wong) says:
>

Dear Eric,

In reply to your Email:- this is what we can do in the field of peptide synthesis
, plus everything else. Just give me a call if you are interested.


Alta Bioscience, the essentials:-

Synthetic methods
Oligonucleotide synthesis		
*	Rapid synthesis of purified oligos ready for use in PCR or 
	sequencing.
*	Synthesis on 0.2 and 1.0 micromole scales.
*	Larger scales of synthesis.
*	Mixed base oligos.
*	Doped oligos.
*	Fluorescein or Biotin tags at any position.
*	Phosphorothioate oligos.
*	Oligos coupled to solid supports.
*	Optional reverse phase cartridge purification.

Peptide synthesis	
*	Laboratory scale synthesis, 50 to 500 micromole amounts of 
	peptides. 
*	Multiple peptide synthesis: 30 micromoles, 12 peptides 
	simultaneously.
*	Multiple peptide synthesis: 5 micromoles, 48 peptides 
	simultaneously.
*	Multiple antigenic peptides, MAPs, (octomeric peptides).
*	Peptides supplied crude or purified.
*	Quality control includes amino acid analysis, HPLC, mass 
	spectrometry.


Sequencing techniques
DNA sequencing	
*	Approximately 400 bases per reaction, depending on template 
	quality.
*	Sequencing of plasmids or PCR products.
*	Can use ordinary primers.
*	Only needs 1 micro gramme of template, (for a plasmid).
*	Data presented as hard copy or in electronic form.

Protein micro sequencing	
*	High sensitivity, capable of sequencing at the pico mole level.
*	Can sequence proteins or peptides.
*	Can sequence either proteins in solution or on PVDF blots.
*	Radio sequencing available.
*	Rapid return of data.

Analytical methods
Amino acid analysis	
*	Amino acid analysis on virtually any type of material.
*	Separation of either protein hydrolysates or biological fluids.
*	All sample preparation included.
*	Choice of detection systems.


Laser desorption mass analysis	
*	Suitable for a wide range of molecular weights of up to 200kDa.
*	Capable of processing large numbers of samples.
*	Picomole sensitivity.
*	Mass measurement accurate to *0.04%.


High Performance Liquid Chromatography	
*	Range of columns available.
*	Choice of detectors, UV, RI, fluorescence, electrochemical.
*	Analytical or semi preparative scale of separations.





For further details on these methods please contact the Director:-

Dr. John E. Fox
School of Biochemistry
The University of Birmingham
Edgbaston
Birmingham
U.K.

Tel     021 414 5450               
Fax     021 414 3376





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