Endoproteinase Asp-N

Andrew, Tel. +39-6-91093434 WALLACE at IRBM.IT
Fri May 13 12:25:35 EST 1994


In Article-Number: bionet.molbio.proteins Msg # 1498
it was written.

>In article <2qu2mu$mn7 at senator-bedfellow.MIT.EDU> jzaia at athena.mit.edu (Joseph Zaia) writes:
>>Does anyone know of a supplier for milligram amounts of endoproteinase
>>asp-N?  ......  Less-than-sequencing grade will suffice for my
>>purpose.  Thank you.
>>

>I do beleive that endoproteinase Asp-N is just a highly purified version of
>V8 protease from staph. aureus. This is available in larger quantities.

>Hope this helps.


>.....d

I think I have to correct you on this. Asp-N and V8 protease are NOT the same
enzyme. Asp-N comes from Pseudomonas fragi. Staph. aureus V8 protease has a
different substrate specificity and is sold commercially as Glu-C by one 
supplier. 

As regards the original question, sorry I don't know a cheap source of Asp-N.
Maybe you can grow the P. fragi strain and isolate it yourself? See
Drapeau, G.R. (1980) J. Biol. Chem. 255,839 for more info.

Andrew Wallace, IRBM, Pomezia, Italy
"IRBM - The home of the Minibody"
wallace at irbm.it



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