Help with Prot.-DNA interactions

Robert Means remeans at husc9.harvard.edu
Fri May 13 10:11:24 EST 1994


Subject: Help with Prot.-DNA interactions
Newsgroups: bionet.molbio.proteins
Summary: 
Keywords: 

Hello,
	I have a problem that I'm hoping that some of you can help me 
with, thus saving me a great deal of legwork in an unfamiliar subject. 
While looking at something completely different, we engineered a purine 
to pyrimidine change into a stretch of DNA that is involved with 
interactions with a certain protein. (sorry to be so circumspect)
At any point, we weren't sure what section of this protein was 
interacting with the DNA and to make a long story short, we are getting a 
compensatory mutation in the protein which causes a Val to Ile change. 
While this change is fairly conservative, I was wondering if anybody out 
there could make any suggestions about what this change might mean. 
Amoung DNA-protein interactions, are there any noted preferences for a 
Val over Ile when interacting with a purine, or vise-versa? Even if no 
one out there can give me a dirrect answer could somebody give a 
suggestion as to how I could screen a large number of DNA-interacting 
proteins for possible clues as to what is going on.

						Thanks in advance,
							Bob Means

New England Regional Primate Research Center...Where the primates have 
better living conditions than the grad. students.

"Why don't activists ever picket for better living conditions for 
me?"--any impoverished grad student





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