Agarose gels for protein purification?
Chandra at mbcg.uchc.edu
Sat May 14 12:58:55 EST 1994
In article <berk-050594154934 at koniskyj3.life.uiuc.edu>
berk at uxa.cso.uiuc.edu (Holger Berk) writes:
> My question THIS time is about using agarose gel electrophoresis for the
> purification of proteins. Has anyone out there had any experience with
> this procedure? Is it possible to detect the proteins in the agarose gel
> by coomassie blue staining, or do you have to transfer the protiens to a
> membrane first? Also, is contamination with proteins present in the
> agarose itself a problem? Any references anyone could send me about this
> procedure would be very greatly appreciated!
> Thank you,
> Ed Beaty
> Ed_Beaty at qms1.life.uiuc.edu
In the past, I have used agarose gels to separate and analyze (by
coomassie) a very large protein subunit (500,000 M.Wt.) under native
conditions. I wonder why that method couldn't be scaled up. The
company FMC has several grades of agrose and I found one of them
suitable for protein work (Diclaimer: I am in no way associated with
that company). Give it a shot.
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