E. coli and SDS-PAGE

Chong-Yee Khoo cyk10 at cus.cam.ac.uk
Thu May 19 20:41:57 EST 1994


Why don't you try sonicating to excess to break up the chromosomal DNA?
Alternatively, you could pass the extract several times through a 24
gauge needle. Shearing the DNA should give a considerable decrease in
viscosity.
Strangely enough, boiling _does_ seem to help, in my hands too...

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Chong-Yee Khoo                     E-mail: cyk10 at cus.cam.ac.uk
Wellcome/CRC Institute             Talk:   cyk10 at cy-mac.welc.cam.ac.uk
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