two-hybrid Gal4 binding domain vector

Kevin Kraus krauske at MARTIN.LUTHER.EDU
Wed May 25 16:46:31 EST 1994

I am interested in using the two-hybrid system to search for proteins which 
interact with the protein I study.  The target protein in this case is secreted.
I have two questions concerning this situation.  (1) Is there available a Gal4 
DNA binding domain vector which adds a nuclear localization signal to the fusion
protein? (2) Does the library need to be constructed in an activation domain 
plasmid which also has nuclear targetting adaptations or does the activation 
domain of Gal4 provide the localization already? Thanks, Kevin.

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