In article <38ks3c$r5e at nuscc.nus.sg>, mcbtansh at leonis.nus.sg (Tan Shyh Han) writes: yes, I do, what do you want to know, To get YY1 expressed well, use the Qiagen pQ vectors. they express well, A slight change, use only 6M guandine-hcl, if any questions, email me at kravetz at sluvca.slu.edu andy