AatII cutting

Nora Plesofsky-Vig npv at MANI.CBS.UMN.EDU
Wed Nov 2 15:26:07 EST 1994

In response to the question of whether AatII is a problem as a restriction enzyme, I have had  
very good luck in using it for cloning, except when the enzyme was old. With relatively fresh  
AatII from Promega, the enzyme cut the pGEM plasmid and the inserted DNA fine.

Nora Plesofsky-Vig
University of Minnesota
nora at molbio.cbs.umn.edu

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