Site Directed Mutagenesis - Current Limitations

Tim Buss tbuss at fred.fhcrc.org
Wed Nov 9 12:40:48 EST 1994


Site Directed Mutagenesis - Current Limitations

In article <gddavis-081194112226 at ppp200.modems.uoknor.edu> Greg Davis,
gddavis at essex.ecn.uoknor.edu writes:
>1.  What are largest synthetic oligonucleotides that can be synthesized 
>    for use as primers without significant sequence errors occurring
>    during their synthesis?  (~60 bases is what I've previously heard.)


I've synthesised oligos up to 130 bases on ABI 394's and 380B's without
problems. I do recommend, though, gel purifying these as the amount of
full length product is relatively low.

Tim.



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