Non-isotopic Western Blots-Best
valeryt at ag.arizona.edu
Fri Nov 18 11:54:00 EST 1994
In article <Cz9JCJ.4p9 at ncifcrf.gov>, reming at ncifcrf.gov (Mary Remington) says:
> Our laboratory will be doing Western blots in a BSL 3 and it would
> simplify our lives greatly to use a non-isotopic method. I would
> appreciate any thoughts on the kits on the market for this purpose.
> Many thanks, Mary
In my lab we have been using chemiluminescent detection for western blots for
about the past 1 1/2 years with good success. We have used the kits from
DuPont NEN and from Boehringer Mannheim. Both work well and are about the
same price/blot. We have not tried the Amersham kit only because it costs
more. It takes a little more work to develop the procedure than using the
colorimetric reagents because of the sensitivity, but once the correct Ab
dilutions are found, the procedure is quite easy. Also there is no problem
with waste disposal as when I-125 is used. The sensitivity I have
experienced is in the sub-nanogram range.
I hope this helps. Good luck.
Sr. Research Specialist
Muscle Biology Group
University of Arizona
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