beta-galactosidase

Mike Dyall-Smith mikeds at ariel.ucs.unimelb.EDU.AU
Tue Nov 29 23:20:01 EST 1994


        We have isolated a beta-galactosidase
-it cleaves X-gal and ONPG. The host bacterium
does not grow on lactose and lactose is not
expected in their normal environment. So our
question is: What is the best way to determine the
substrate specificity?? We are particularly interested
in what the natural substrate could be (whether it
is a catabolic or anabolic enzyme).
Thanks for any help. mike




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