westerns w/ HA-tagged proteins

Ms. Cynthia Bolognese; BIOL; GRAD bolognes at umbc.edu
Tue Sep 6 11:25:37 EST 1994


I'd like some input on westerns with HA-tagged proteins.

I can't detect my HA-tagged protein on a western, nor can I detect the 
positive control. I've tried various ab dilutions and have had no luck. 
Transfer appears to be complete, yet I get no signal from my protein or
positive control, while the 14C standard is very strong. 

Any suggestions?

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Cindy Bolognese		 |						  |
e-mail address:  	 |    Qapla'!  (Klingon for Success!)             |
bolognes at umbc7.umbc.edu	 |						  |
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