HELP WITH Organalles

[Phil Whitehead]

I'm trying to purify some subcellular organales with an apparent molecular
weight of 600 kD. I'd like to be able to follow the purification bylooking
at the intact protein on a gel.
Obviously SDSPAGE is out, but occasionaly on the net people have aluded to
agarose gels can anyone in the know either post their ideas or E-Mail me a
ny proceedures you may have. 
I'm not desperate (yet) but it would be nice to have a few methods up my
sleve
thanks in advance

Phil Whitehead