HELP WITH Organalles

Phil Whitehead p_whitehead at
Tue Sep 6 07:21:08 EST 1994

I'm trying to purify some subcellular organales with an apparent molecular
weight of 600 kD. I'd like to be able to follow the purification bylooking
at the intact protein on a gel.
Obviously SDSPAGE is out, but occasionaly on the net people have aluded to
agarose gels can anyone in the know either post their ideas or E-Mail me a
ny proceedures you may have. 
I'm not desperate (yet) but it would be nice to have a few methods up my
thanks in advance

Phil Whitehead

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