Proteins over 400 KDa

[slf8 at po.cwru.edu]

Dear Protein Researchers,
	Our lab is working with a nuclear protein which is over
400 KDa.  We have been running 6% DATD-acrylamide minigels to 
visualize the protein with minimal success. (It just smears.)  I was
wondering if anyone else has had experience in working with proteins
of this size and what they would suggest as far as gel apparatus.
Would horizontal agarose gels be better, and if so, what type of equipment
will we need?
	Thanks in advance.

Stephanie L. Fox
slf8 at po.cwru.edu
Graduate Student, Dept. of Genetics
Case Western Reserve University

 

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