unwanted ppt in Biorad assay

Hemlata Mistry hm10006 at mole.bio.cam.ac.uk
Thu Sep 8 11:44:32 EST 1994


Help!

I get a large precipitate in a Biorad protein assay.  Maybe my assay
conditions are not right. I have been extracting cAMP from Drosophila eggs
using 6% TCA or hot 0.1M HCl, and saving the pellets containing protein +
eggshell. I resuspend the pellet in deoxycholate, spin down undissolved
stuff and try to assay amount of protein in supernatant. All I have got so
far are massive blue pptes, whose OD I cannot measure! Any suggestions?



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