concentrating proteins

[john markwell]

jbooth at ACS.UCALGARY.CA (Joseph Booth) writes:

>Hi!

STUFF DELETED. . .. Also, any alternative suggestions for a gentle method of 
>concentrating proteins would be appreciated. Thanks.

>Joe

Joe,
One trick for concentrating proteins from the dark ages is to freeze a
tube of the protein at -20C.  After frozen solid, place the tube on 
the lab bench and let thaw undisturbed.  The bottom of the tube will 
contain concentrated protein and the top of the tube will be water.  
In between is a gradient.  You decide how much to discard from the top
and the remaining solution is concentrated protein.  I hope this 
helps.  

John

--
John Markwell			Phone: 402-472-2924
Dept. Biochemistry		FAX:   402-472-7842
University of Nebraska		Internet: markwell at unl.edu
Lincoln, NE  68583-0718