ppt protiens

allera at mailer.meb.uni-bonn.de allera at mailer.meb.uni-bonn.de
Mon Sep 12 09:48:45 EST 1994


In article <magoldstein-2908942114590001 at modem67.ucdavis.edu> magoldstein at ucdavis.edu (Marc A. Goldstein) writes:
>In article <thomas-2908941939110001 at fp1-molbio-19.uoregon.edu>,
>thomas at molbio.uoregon.edu (matt thomas) wrote:
>
>> I am planing to do an experiment where I will end up with a large volume.  I 
>> want to run this on a SDS gel and my lanes will not hold the volume the
>> experiment will generate. I don't care if the proteins get killed.  Does
>> anyone have a good meathod they might suggest?
>
>Dear Thomas,
>
>   What's a large volume, and how large are you're wells?
>
>Some standard thoughts:
>   1.  Concentrate the protein by using small-pore-sized spin filters
>(millipore, amicon, etc..)
>   2.  Precipitate the protein with acetone, or ammonium sulfate or the like.
>   3.  If all else fails, dialyze the salt away and lyophilize the stuff.
>
>Hope this helps.
>________________________
>Marc A. Goldstein
>Section of Plant Biology
>UC Davis
>magoldstein at ucdavis.edu



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