Recommendations for light level protein detection?

[Dr. Dennis Goode]

Often the problem of lack of antibody staining of whole cells or 
fixed sections is one of antibody penetration, especially with 
aldehyde fixation. I'd try fixation with cold methanol at -20 C 
instead of aldehydes, or using a detergent such as 0.2% Triton x-100
after fixation in a low concentration (2% paraformaldehyde or 0.6% 
glutaraldehyde) of the aldehyde fixative. See Harlow and Lane: 
Antibodies A Laboratory Manual (CSH lab press).
Hope this helps.

Dennis Goode 
Goode at Zool.umd.edu