affinity tags

David Mueller muellerd at MIS.FUHSCMS.EDU
Mon Sep 12 15:55:14 EST 1994


Are there any tags that can be used on the amino end
of a protein to aid in its purification  - outside
the His tag for Ni, the Cys tag for Hg and
epitope tags?

I need a third tag and fear that an epitope tag
would not be aceptable because of the cost of the
Ab and the difficulty in removing the Ab from
the tag.  

Thanks in advance.
David Mueller
Dept. Biochemistry
Chicago Medical School




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