dimerization

Nora Vig nora at MOLBIO.CBS.UMN.EDU
Wed Sep 14 11:34:11 EST 1994


A possible way to estimate the dimerization of a protein, if cross-linking is
unsuccessful, would be to separate a protein mixture on a native gel, using
an appropriate acrylamide gradient (e.g. 3-20% for a wide range of sizes). The
gel can be run to equilibrium at 200 V for about 18 hr at 4oC. It is best,
of course, if you have some way to identify your protein. Gel migration is
not the final word on protein size or oligomerization, but it is a reasonable
first step and might indicate if there is a difference between the wild-type
and mutant proteins. Good luck

Nora Plesofsky-Vig
Department of Plant Biology
University of Minnesota
St. Paul, Minn. 55108

nora at molbio.cbs.umn.edu



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