Renaturation of proteins from 8M Urea - references?
David I Resnick
dresnick at athena.mit.edu
Thu Sep 15 12:30:41 EST 1994
I'm expressing a ~130 amino acid protein fragment in E. coli. After
solubilization in 8 M Urea, I purify it using a His6 tag. I then want
to get rid of the urea (and pray that it will fold). Everything I've
tried so far (not many things, but a few) has resulted in
precipitation. Anyone out there know of a comprehensive reference for
this sort of thing? Any ideas???
David Resnick dresnick at athena.mit.edu
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