Renaturation of proteins from 8M Urea - references?

David I Resnick dresnick at
Thu Sep 15 12:30:41 EST 1994


I'm expressing a ~130 amino acid protein fragment in E. coli.  After
solubilization in 8 M Urea, I purify it using a His6 tag.  I then want
to get rid of the urea (and pray that it will fold).  Everything I've
tried so far (not many things, but a few) has resulted in
precipitation.  Anyone out there know of a comprehensive reference for
this sort of thing?  Any ideas???


       David Resnick                           dresnick at      

More information about the Proteins mailing list